Standardization of clinical enzyme assays: a reference method for aspartate and alanine transaminases.

Standardization of clinical enzyme assays: a reference method for aspartate and alanine transaminases.

Standardization of clinical enzyme assays.

During 1969, discussions between the Standards Subcommittee of the Laboratory Equipment and Methods Advisory Group of the Department of Health and Social Security and representatives of professional societies concerned with the assay of enzymes for diagnostic purposes led to the establishment of a working party to consider the current state of accuracy and reliability of diagnostic enzymology a...

A unifying reference system for clinical enzymology: aspartate aminotransferase and the International Clinical Enzyme Scale.

A review of methodology for determining aspartate aminotransferase (ASAT; EC 2.6.1.1), including recent national and international recommendations, indicates that standardization of methodology alone will not bring interlaboratory compatibility of ASAT results. We propose that an additional component to standardization is needed, namely, enzyme reference materials. Furthermore, we suggest that ...

Introducing a Method for Achieving Standardization and Harmonization in Clinical and Research Laboratory Centers

Abstract Background and Objective: Proficiency testing schemes as a part of quality system in clinical and research laboratory centers provides the opportunity to evaluate the quality of test results. In this paper, we try to introduce the proficiency testing schemes as a useful method for achieving standardization and homogenization of test results in clinical and research laboratory center...

Enzymes of Amino Acid Metabolism in Normal Human Skin Ii. Alanine and Aspartate Transaminases*

drogenase in normal human skin. In comparison with glutamate dehydrogenase, which catalyzes reversible oxidative deamination, the transaminases catalyze transfer of amino groups, usually from an amino acid to a ketoacid, without net loss of nitrogen. In other words, transamination represents the intermolecular exchange of amino nitrogen. Transamination plays a significant role in metabolism by ...